Collapsible content

How accurate is Peptest?

Peptest is a highly accurate diagnostic test with performance measures validated in several clinical tests, as indicated below:

Sensitivity: 95%
Specificity: 89%
Pepsin detection limit: 16 nanograms per millmetre (16ng/ml)

Sensitivity is defined as the proportion of people with disease who have a positive test result, ie a highly sensitive test helps rule out disease when the result is negative.

Specificity is defined as the proportion of people without disease who have a negative test result, ie when a highly specific test is positive you can be nearly certain that you actually have the disease.

Peptest will measure the concentration of pepsin in your saliva to show the severity of reflux and give a benchmark to assess the success or failure of any treatment. The detection limit of 16ng/ml is the lowest amount of Pepsin that can be measured.

Should I take the three sample Peptest or the two sample test?

Peptest reflux diagnostic kits are available in a choice of two or three sample tubes. Taking two samples at the right time and according to instructions offers extremely accurate results.However, in addition to collecting two samples when you are symptomatic, a third tube can help identify if you are prone to supine (or nocturnal) reflux. When someone suffers from supine reflux, tissue damage tends to be more severe and far more injurious than daytime reflux.Collecting three samples will provide you with our most comprehensive testing and results service and will enable you and your doctor to tailor the treatment and management of your reflux symptoms.

Check out the instructions for the 3-sample collection procedure.

When to take samples? 

The Saliva Collection Form Booklet provided with your order offers detailed guidance on when to collect your samples. In summary, if you experience symptoms or suspect a reflux episode, please provide your saliva samples at that time. If you're unsure, collect samples one hour after your main meals. Additionally, it's recommended to collect a third sample upon waking up.

Download the instructions for our 3-sample collection procedure. 

 How to provide your saliva samples correctly?

To collect a saliva sample, start by clearing your throat with a cough and then spit the resulting saliva into the collection tube provided. Remember to note down the time of collection and any specific symptoms you may be experiencing.For a visual demonstration of the correct sample collection method for Peptest analysis, we recommend watching our instructional video.

Should I stop taking medication such as PPI therapy, antacids, Gaviscon or H2 receptor antagonists prior to taking samples?

We recommend that you stop taking antacids and raft-forming/ alginate suspensions such as Gaviscon for 48 hours prior to collecting samples for Peptest analysis.

You do not have to stop PPI or H2 receptor antagonist therapy as Peptest will still detect pepsin in the presence of these if pepsin is being refluxed.

Read advice on which medications will affect your Peptest results.

Should I stop drinking alkaline water before taking Peptest?

Yes. Alkaline water (pH 8.8) has been demonstrated to denature pepsin in in-vitro studies. Although we know of no published data that exists to demonstrate whether or not alkaline water has any impact on reflux symptoms we recommend that alkaline water is avoided for 48 hours prior to taking the samples for Peptest.

Find out more about alkaline water.

How long does it take to get my results?

Once we receive your  saliva samples, it takes around 20 minutes for our technicians to analyse these in the Peptest laboratory.

We will share results with you on the same day as testing your sample – typically this is within 48 hours of a sample arriving at the lab.

What should I do once I get my diagnosis?

Once your saliva samples have been returned to the Peptest labs for analysis you will receive the results report within two days indicating whether each sample is positive or negative. If positive you will be given a reading of the concentration of pepsin in the sample.

You should then discuss the next steps with your healthcare team. The exact treatment pathway you follow will depend on your symptoms, the results of your Peptest® analysis and any other relevant information your healthcare team possess.

Can I have LPR symptoms but not have reflux?

A patient can present with laryngopharangeal reflux (LPR) symptoms which are not necessarily caused by reflux and this is the reason why a negative pepsin result can be as important as a positive pepsin result to help us identity the reasons why patients are experiencing symptoms at that time. If you have a positive pepsin result this indicates that reflux has occurred and the reflux should be treated appropriately.

A negative pepsin result in a patient with LPR like symptoms is far more challenging. The symptoms may be due to i) a previous reflux event which has damaged the sensitive epithelium cell layer of the throat, vocal cords, airways ii) this damaged area has allowed bacteria to colonise which will cause inflammation and irritation and possibly needs to be treated with antibiotics on anti-inflammatory type medication iii) the symptoms might be the result of an allergy or food intolerance and this will need investigating iv) the damage to the delicate epithelial cell lining might have been caused by smoking or alcohol or by insulting the cell lining by drinking excessively hot or acidic drinks.

One suggestion if symptoms persist and reflux has been ruled out by a negative Peptest result is for the patient to undergo a course of vocal/throat hygiene. A good ENT (Ears,Nose and Throat) specialist can advise here or even better get in touch with a Speech Language Therapist attached to the ENT clinic.

Read more on why LPR (silent reflux) symptoms may not be reflux.

Is Peptest available on the NHS list and DHB list ?

We’re working hard on making Peptest available in the NHS and have made great progress with ongoing work at nine hospitals across the UK and we will start to work with DHBs in New Zealand.

We are also work closely with medical company BiohitUK, our UK distributor, to raise awareness of our reflux diagnostic service.

We would be happy to put you in touch with a UK consultant that is currently using Peptest. If you would like to email us on info@maab-group.com our team will be ready to help. 

Do you deliver to international addresses?

Peptest is available to most international countries.

If you live outside the New Zealand, we will send your kit using FedEx international priority. We also send you a FedEx return pack with pre-printed airway bill so that your return will be just as quick.

If you live in the US, please visit www.pepsincheck.com to place your order

If you live in the UK and Europe please visit www.peptest.co.uk to place your order